Species reactivity: Human
Published species: Human, Mouse
Host / isotype: Mouse / IgG1, kappa
Concentration: 0.5mg / ml
Purification: Affinity chromatography
Storage buffer: PBS, pH 7.2
Contains: 0.09% sodium azide
Storage conditions: 4 ° C
Description: HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as common leukocyte antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in lymphocyte activation.
Informed Applications: The HI30 antibody has been reported to be used in flow cytometric analysis and immunohistochemical staining.
CD45 (LCA, common leukocyte antigen) is a receptor-like protein tyrosine phosphatase (PTP) that is ubiquitously expressed on all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins on lymphocytes. CD45 is absent in non-hematopoietic cell lines, normal and malignant non-hematopoietic tissues. The CD45 glycoprotein is crucial in lymphocyte development and antigen signaling and acts as an important regulator of Src family kinases.
The CD45 protein exists as multiple isoforms as a result of alternative splicing, differing in their extracellular domains but sharing identical cytoplasmic and transmembrane domains. CD45RA is an isoform of the CD45 complex and has restricted expression among different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into glycosphingolipid-enriched membrane domains and their expression depends on the cell type and the physiological state of the cell.
CD45 has been shown to be an essential regulator of T and B cell antigen receptor signaling and suppress JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating the integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis, and the inhibition or up-regulation of various immune functions.