Next Gen Sequencing Blog Purified Native dsDNA, Calf Thymus

Purified Native dsDNA, Calf Thymus



Properties

Quality level: 100

Form: lyophilized

Packaging: 10mg pack

mfr. Not.: Roche

Sent in: wet ice

Storage temperature: 2-8 ° C

Related categories

Roche® Life Science Products

General description

A mix of histones H1, H2A, H2B, H3, and H4, isolated from the calf thymus. Histones are a group of DNA-binding proteins that are characterized by relatively high levels of lysine and arginine. Five different histone fractions have been isolated and characterized. These are called H1, H2A, H2B, H3, and H4. Fraction H1 is rich in lysine, while H2A and H2B are slightly rich in lysine, while fractions H3 and H4 are rich in arginine. The molecular weights of histones are approximately 11 to 21 kDa depending on the fraction.

Request

Histone from calf thymus has been used:

  • In vitro kinase assay to check the phosphorylation of histones by protein kinase A.
  • to check the in vitro methyltransferase activity of the arginine methyltransferase proteins from Oryza sativa (OsPRMT).
  • in anti-histone ELISA.

Biochemical / Physiological Actions

Histones play an important role in the organization and modification of chromatin. The nucleosome is the basic unit of chromatin. It is composed of two molecules, each of the histones H2A, H2B, H3, and H4, enveloped with approximately 146 bp of DNA. Histone H1 binds to nucleosomes at DNA entry and exit sites and stabilizes them.

Physical form

Lyophilized (a natural mixture of histones H1, H2A, H2B, H3, and H4).

Preparation note

  • Working concentration: 1 to 5 mg / ml.
  • Working solution: The recommended solvent is double distilled water.
  • Storage conditions (working solution): The aqueous solution is stable at -15 to -25 ° C for several months.
  • Avoid repeated freezing and thawing!

Other notes

For life science research only. It should not be used in diagnostic procedures.

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