Next Gen Sequencing Blog QuantiChrom™ Creatinine Assay Kit

QuantiChrom™ Creatinine Assay Kit



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For the quantitative determination of creatinine and the evaluation of the effects of drugs on creatinine metabolism.

Key features

  • Sensitive and precise. Use 30 μl samples. Detection limit 0.10 mg / dL (8 μM) creatinine in 96-well plate assay.
  • Simple and high performance. The procedure involves the addition of a single working reagent and incubation for 5 min. It can be automated as a high throughput assay for thousands of samples per day.
  • Greater stability and versatility of reagents. The optimized formulation has greatly improved signal and reagent stability. Assays can be performed in 96-well plates or cuvettes.
  • Low interference in biological samples. No pretreatments are needed. Assays can be performed directly on raw biological samples.

Method: OD510nm

Samples

Urine, serum, plasma, and biological preparations

Species: All

Size: 500 tests

Detection limit: 0.1 mg / dL (8 μM)

Duration: 12 months

More details

Creatinine is synthesized in the body at a fairly constant rate from creatine, which is produced during muscle contractions from creatine phosphate. In the blood, creatinine is removed by filtration through the glomeruli of the kidney and is secreted into the urine. In healthy individuals, creatinine secretion is independent of diet and is fairly constant. The creatinine clearance test has become one of the most sensitive tests for measuring the glomerular filtration rate.

In kidney disease, the blood creatinine levels are elevated, while the creatinine clearance rate and thus the urine levels are decreased. The creatinine test is the most widely used to assess kidney function. Simple, straightforward, and automation-ready procedures for measuring creatinine concentration in biological samples are becoming popular in drug discovery and research.

The BioAssay Systems Creatinine Test Kit is designed to measure creatinine directly in biological samples without any pre-treatment. Jaffe’s improved method uses picrate which forms a red complex with creatinine. The intensity of the color, measured at 510 nm, is directly proportional to the creatinine concentration in the sample. The optimized formulation substantially reduces the interference of substances in the raw sample.

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